Welcome to EpifluorescenceMicroscopes.com! You have found the source of low cost quality discount epifluorescence microscopes. Typically Costing Thousands More, We Have Affordable Solutions for Your Fluorecence Microscopy Needs! Available in Both Inverted and Upright Models! Ideal for Advanced Biomedical Research, Research in Biology, Cytology, Oncology, Haemotology, Genetics, Immunology, Environmental Protection, Microchemistry, Analysis of Sedimentary Rock, and Inspection of Impurities in Semiconductors. Welcome to EpifluorescenceMicroscopes.com! You have found the source of low cost quality discount epifluorescence microscopes. Typically Costing Thousands More, We Have Affordable Solutions for Your Fluorecence Microscopy Needs! Available in Both Inverted and Upright Models! Ideal for Advanced Biomedical Research, Research in Biology, Cytology, Oncology, Haemotology, Genetics, Immunology, Environmental Protection, Microchemistry, Analysis of Sedimentary Rock, and Inspection of Impurities in Semiconductors. Contact Epifluorescence Microscopes
 
Model: ef0089000n
EPI - FLUORESCENCE INVERTED
TISSUE CULTURE MICROSCOPE
+ PHASE CONTRAST MICROSCOPY
+CCD CAMERA +USB CAMERA +INFINITY

Price: $ 3,699.98
Shipping/Handling/Insurance: $ 74.98
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
Product Overview
  • High Grade Epi-Fluorescence Trinocular Inverted Tissue Culture Microscope!
  • Microscopy Techniques Included: Incident Light Fluorescence Excitation, Phase Contrast Illumination, Brightfield Transmitted Light.
  • Looking for an Inverted Fluorescence Microscope? This is a high grade system, capable of excellent quality imaging.
  • A Clear and High Contrast Epi-Fluorescence Image is produced with the incident light excitation from the Mercury bulb.
  • Ideal for Study of Tissue Cultures, Advanced Biomedical Research, Research in Biology, Cytology, Oncology, Haemotology, Genetics, Immunology, Environmental Protection, and Microchemistry.
  • Great for Viewing Live Biological Tissue Cultures in Petri Dishes!
  • Inverted Design allows for Viewing from the Bottom of the Dish. Perfect for Examining Liquid Deposits!
  • Includes Phase Contrast Kit for Observing Translucent Low Contrast Specimens!
  • Also Capable of Transmitted Light, Bright-Field Microscopy
  • Compare to Similar Units costing $20,000 with Name Brands!
  • Excellent Bright and Clear Images through High Quality Optics.
 Trinocular Photography Port
  • Photograph Your Own Specimens!
  • Have Your Own Complete Digital Video Microscopy System!
  • Capture Images With Your Computer!
  • Display Images on a High Resolution CCTV Monitor or Television!
  • Videotape What You See Under the Microscope!
  • Versatility - Included Trinocular Port Greatly Expands the Usefulness adding Photographic/Video Capability!
  • Other (not included) accessories may be required for connecting digital/film cameras, CCD cameras and Video cameras. Call or Email us your specific requirements.
 Special Features
  • Higher grade Infinity Corrected optical system. This type of optics is more expensive than normal fixed tube length optics. It is found only on higher grade compound light microscopes.
  • Features 30mm Eyepiece Ocular Tubes! Most compound scopes are 23mm. The larger size allows for less eye strain, much easier viewing, and a larger and better quality image! After looking into these awesome eyepieces, you would never want a smaller 23mm ocular! Before buying a different scope, ask if it has the cheap 23mm oculars!
  • Unique Ability to have Image in Both Oculars and Photo Port Simultaneously. This is Perfect for Training as the Binocular Eyepieces can be viewed by the Instructor, and Everybody Else can Watch the viewing screen. Most Trinocular Scopes lose light in one or both oculars when light is diverted to the photo port.
 Eyepieces and Magnification
  • Four Fluorescence (and Brightfield Transmitted Light) Magnifications: 40x, 100x, 200x, and 400x.
  • Four Brightfield Long Working Distance PLAN Infinity Corrected DIN Objectives: 4x (0.10 NA), 10x (0.25 NA), 20x (0.40 NA), 40x (0.60 NA).
  • Three Phase Contrast Magnifications: 100x, 200x, and 400x.
  • Three Phase Contrast Objectives: 10x, 20x and 40x. These are the same as the brightfield objectives. They are used for both brightfield microscopy and phase contrast microscopy.
  • Quintuple objective nosepiece holds up to five objectives.
  • Objectives are designed for being able to view the culture specimen through the bottom of the petri dish or glass flask.
  • Eyepiece Set Included: 30mm diameter wide field 10x / 22mm Field. High eyepoint eyepieces, great for users with eyeglasses. Includes rubber eyeguards for blocking stray light.
 Head - Interpupillary Distance - Diopter
  • 45 Degree Inclined Trinocular Head.
  • Adjusts to the Distance Between your Eyes: 50 to 75mm InterPupillary Distance.
  • Diopter Adjustment to Correct for Your Specific Vision Needs.
 Condenser - Iris - Filters
  • Brightfield - Phase Contrast Illumination System Consisting of the Following:
  • Above-Stage Long Working Distance (75mm from stage to bottom of condenser) Condenser, with iris diaphragm and filter holder on slider bar.
  • Phase Annulus Rings for 10x, 20x, and 40x built into phase slider bars. Each slider bar has open hole for brightfield setting. This same hole can be used for placing a brightfield color filter.
  • Ring Centering Knobs for Precise Alignment of Phase Rings for Optimal Phase Contrast Performance.
  • Four brightfield filters included: Slight Blue (for daylight color correction), Green, Yellow, and Frosted White.
  • Trinocular photography port has a color corrected daylight filter that can be inserted into light path or removed.
  • Fluorescence Microscopy Illumination system with two dichroic fluorescence filters (blue and green). The fluorescence filter cubes are mounted on a slider bar assembly so either can be easily selected. It can be set to middle position with no filter cube for brightfield microscopy. Note: If no image is obtained, or image is only partial, be sure slider bar is not partially seated between settings. For brightfield, no image will be obtained if fluorescence filter cube is in light path.
 Illumination
  • Above Stage Transmitted Light Source: Variable Intensity 12V/30W Halogen Illumination.
 Stage Specifications
  • Large Mechanical X-Y Movable Stage. Stage dimensions: X = 172mm, Y = 240mm.
  • Low Position Coaxial X-Y Specimen Holder Movement Knobs - Range of Movement: 70mm (X-Direction) x 120mm (Y-Direction).
  • Includes several types of holders for slides, petri dishes, and flasks.
  • Stage does not move up and down. Instead, the objective turret moves up and down to focus.
 Focusing
  • Coaxial Fine and Coarse Focusing Knobs on Both Sides of Microscope.
  • For Optimal Performance when Focusing, the Objectives move Up/Down Instead of the Stage.
  • Fine Adjustment Division: 0.002mm.
  • Adjustable Lock Ring Stop to Limit Coarse Range to Protect Objectives and Specimens.
  • Tension Adjustable Coarse Focusing.
 Frame - Base - Size - Weight
  • Total Overall Height of Microscope: 490mm.
  • Instrument Weight: 11.5 Kg.
 Aluminum Carrying Case
  • Orange eye guard shield on Microscope Front.
  • Includes: Extra Mercury Bulb (for fluorescence observation).
  • Includes: Dust Cover, Extra 12V/30W Bulb (for brightfield), and Extra Fuse.
  • Includes phase contrast telescope for centering of phase annulus rings with the phase objectives.
 Additional Information
  • Manufactured under ISO: 9001 Standards.
  • Precision Ground Glass Lens.
  • Quality Construction, Metal Frame.
  • 110 VAC (email about 220V availability).
  • 100% Brand New!
 1 Year Manufacturer's Warranty
  • The warranty covers manufacturing defects. Abuse or damage due to dropping is not covered.
  • If your system needs repair, simply let us know and we will see that it is back in service ASAP.
 
  • A conventional microscope uses regular light to illuminate a specimen and create a magnified image from that light. A fluorescence microscope uses a special high intensity light to illuminate the specimen. The light excites fluorescent particles which in turn emit light of a longer wavelength. It is this secondary light being emitted that is collected and used to produce a magnified image of the specimen. The original high intensity light is not magnified and is filtered out of the final magnified image.
  • In a conventional microscope, the condenser lens focuses the light source and the objective lens magnifies the image. The fluorescence microscope objective lens not only magnifies the image, but first, it focuses the high intensity (excitation) light on the specimen. Both the excitation light and the emission light travel through the same objective. This is called epi-fluorescence.
  • The excitation light is filtered into a light of a wavelength that is desired to illuminate the specimen, and all other wavelength light is blocked. A dichromatic beam splitter (mirror) is used to do this. The excitation light path is originally perpendicular to the objective. In order for the light to reach the specimen, it must be reflected 90 degrees into the objective. The beam splitter is on a 45 degree angle to the incoming excitation light. It reflects the shorter wavelength (desired excitation light) light 90 degrees through the objective and onto the specimen. The longer wavelength light passes through and is not reflected to the specimen.
  • The light coming from the specimen (both emitted from specimen and reflected excitation light) is then filtered again so only the light from the fluorescence emission coming from the specimen is passed and used to create the final image. The light creating the final image should mainly be the emission light with minimal background illumination excitation light. The dichromatic beam splitter is again used for this since it allows the longer wavelength emitted light to pass straight through so it can be used for image formation. It reflects the shorter wavelength light in a different direction, so it is not used for final image formation. These filters and mirrors are typically housed within the fluorescence cube assembly.
  • Review the fluorescing specimen photos in this ad. The visible image is really the specimen as it is fluorescing (light emitted from the specimen). It is not normal light that is being reflected from or transmitted light passed through the specimen. This is not Bright-Field Microscopy which is completely different as it produces a strong bright image from normal light that is transmitted through the specimen or reflected off the specimen.
  • Warning: Be careful about buying used equipment that might have scratched optics, delamination, missing/broken parts, dust/dirt on internal optics, head out of alignment (slight double vision, common!), bio-contamination, degradation of lubrication, and impossible-to-find or expensive replacement components on obsolete models. Just like buying an old car, the high cost of maintenance alone must be considered when making a purchase. If you buy used, what will you do if the light goes out and it's not a burnt bulb? Microscope Repair is VERY Expensive!
  • This is a Sophisticated Instrument. We have found that most customers call us after the sale for questions and support for this item. Beware of buying from an inexperienced peddler who knows little about their product. For example, we've seen others selling darkfield microscopes and actually calling them phase contrast microscopes because they didn't know the difference! What will you do if you buy a scope from an inexperienced salesman and don't obtain the quality images that you require? Who can you turn to for help? We have a fully trained microscopy support staff willing to assist you with your needs. All our photos are taken in-house by the same staff available to talk to you today. They are also available to assist you AFTER the purchase when you receive your equipment and are learning its operation.
 
  • BONUS: Includes an Aluminum Carrying Case with Precut Foam for safe transportation of your precision instrument.
  Product
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY EPI - FLUORESCENCE INVERTED TISSUE CULTURE MICROSCOPE + PHASE CONTRAST MICROSCOPY +CCD CAMERA +USB CAMERA +INFINITY
 

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